LOCUS       pUC-frGFP              3236 bp    DNA     circular     16-04-2021
SOURCE
  ORGANISM
FEATURES             Location/Qualifiers
     promoter        72..90
                     /label="T7 promoter"
     CDS             152..253
                     /label="NHis(NHH) tag"
     CDS             254..274
                     /label="TEV recognition seq."
     CDS             275..292
                     /label="NL1"
     CDS             293..1006
                     /label="frGFP"
     CDS             1007..1024
                     /label="CL1"
     terminator      1106..1153
                     /label="T7 teminator"
     rep_origin      1489..2077
                     /label="pMB1 ori"
     CDS             complement(2248..3108)
                     /label="bla"
     promoter        complement(3109..3213)
                     /label="bla promoter"
ORIGIN
        1 agcggataac aatttcacac aggaaacagc tatgaccatg attacgaatt cagatctcga 
       61 tcccgcgaaa ttaatacgac tcactatagg gagaccacaa cggtttccct ctagaaataa 
      121 ttttgtttaa ctttaagaag gagatataca tatgaaagat catctcatcc acaatcatca 
      181 caaagaggag cacgctcatg cccacaacaa ggattacgat atcccaacta ctcatcacgc 
      241 tcatcacagt actgagaacc tgtacttcca gggatccagc ggctcctcgg gaatgtcgaa 
      301 aggcgaagaa ctgtttactg gtgtagttcc gattctggtg gagttagacg gtgacgttaa 
      361 cggtcacaaa ttcagtgttt ccggtgaagg tgaaggagac gctacttacg gtaagttaac 
      421 cctgaaattc atctgtacaa ccggtaaact gccggtacct tggcctacgc tggtgactac 
      481 cctgacttac ggtgtacagt gctttagccg ttatcccgat cacatgaaac gtcacgactt 
      541 ctttaaatct gctatgccag aaggttacgt gcaggaacgt accatttcgt ttaaagacga 
      601 cggtaactac aaaacccgtg cagaagtcaa attcgaaggt gatactctgg tgaatcggat 
      661 tgaactcaaa gggatcgact ttaaggagga cggtaatatt ctgggccata aactcgagta 
      721 caactacaac agccacaacg tgtatatcac cgcggataag cagaagaacg gcatcaaagc 
      781 gaacttcaaa attcgccata acatcgaaga cggttctgtc caacttgccg atcattacca 
      841 gcagaataca ccgattggtg acggacctgt tttgcttccg gacaaccatt acctgtctac 
      901 gcaatcagcc ttgtccaaag acccgaacga aaaacgcgac cacatggtct tactggagtt 
      961 tgttacggca gctggcatta cgcacggtat ggacgagctg tataaatcgg ggccctcgtc 
     1021 aggataataa tccggtaccc cgcccacggc tgctaacaaa gcccgaaagg aagctgagtt 
     1081 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt 
     1141 gaggggtttt ttgctgaaag gaggaactat atccggataa cctcgagctg caggcatgca 
     1201 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgctgcatta 
     1261 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 
     1321 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 
     1381 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 
     1441 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 
     1501 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 
     1561 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 
     1621 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 
     1681 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 
     1741 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 
     1801 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 
     1861 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 
     1921 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 
     1981 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 
     2041 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 
     2101 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 
     2161 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 
     2221 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 
     2281 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 
     2341 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 
     2401 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 
     2461 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 
     2521 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 
     2581 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 
     2641 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 
     2701 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 
     2761 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 
     2821 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 
     2881 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 
     2941 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 
     3001 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 
     3061 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 
     3121 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 
     3181 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//