Cell Free Protein Expression kit "Musaibo Kun"

Home Cell Free Protein Expression kit "Musaibo Kun"

Cell Free Protein Expression kit
"Musaibo Kun"

Musaibo Kun N series

Musaibo Kun Start

Musaibo Kun SI

Musaibo Kun SI SS

Musaibo Kun Quick

Stable Isotope Labeled Amino Acid Mixture for Musaibo Kun

Technical information

Stable-Isotope Labeling Using Cell-free Protein Synthesis

Takanori Kigawa, Ph.D
RIKEN Systems and Structural /Biology Center
1-7-22 Suehiro-cho Tsurumi,
Yokohama, 230-0045

Cell free protein synthesis is a technology to synthesize proteins in vitro by cell extract supplemented with amino acids, target protein gene, and energy regeneration system. RIKEN's advanced E. coli based cell free protein synthesis technology is now available as Musaibo Kun kits. The kit enables rapid and easy protein synthesis with high isotope labeling efficiency.

* This product was developed with the support of the Japan Science and Technology Agency's "Industry-Academia Joint Seeds Innovation Project."

We highly recommend these kits for first trial. The kit can express intracellular proteins, secretory proteins, and membrane proteins. Proteins can be easily synthesized by adding template DNA and incubating for 1-16 hours because the reaction solution contains required components for protein synthesis including amino acids.

Product information / Kit Contents

Product No.	Product Name	Target Proteins	Additive of membrane proteins	Premix reaction solution	Premix dialysis solution	Control DNA pUC-frGFP^*1	Control DNA pUC-BAP^*2	Dialysis Unit (MWCO: 12～14 kDa)	Dialysis membrane cup (MWCO: 15 kDa)	shaker ball
Product No.A235-0300	Product NameMusaibo Kun N Mini	Target ProteinsIntracellular proteins	Additive of membrane proteins○	Premix reaction solution50 μL reaction × 18	Premix dialysis solution-	Control DNA pUC-frGFP^*150 ng/μL × 50 μL	Control DNA pUC-BAP^*2-	Dialysis Unit (MWCO: 12～14 kDa)-	Dialysis membrane cup (MWCO: 15 kDa)-	shaker ball-
Product No.A236-0301	Product NameMusaibo Kun N 100	Target ProteinsIntracellular proteins	Additive of membrane proteins◎	Premix reaction solution100 μL reaction × 8	Premix dialysis solution1 mL dialysis × 8	Control DNA pUC-frGFP^*150 ng/μL × 50 μL	Control DNA pUC-BAP^*2-	Dialysis Unit (MWCO: 12～14 kDa)8 sets	Dialysis membrane cup (MWCO: 15 kDa)-	shaker ball-
Product No.A237-0302	Product NameMusaibo Kun N 1000	Target ProteinsIntracellular proteins	Additive of membrane proteins◎	Premix reaction solution1 mL reaction × 1	Premix dialysis solution10 mL dialysis × 1	Control DNA pUC-frGFP^*150 ng/μL × 50 μL	Control DNA pUC-BAP^*2-	Dialysis Unit (MWCO: 12～14 kDa)-	Dialysis membrane cup (MWCO: 15 kDa)1 set	shaker ball1 set
Product No.A238-0303	Product NameMusaibo Kun N Mini SS	Target ProteinsSecretory Proteins	Additive of membrane proteins○	Premix reaction solution50 μL reaction × 18	Premix dialysis solution-	Control DNA pUC-frGFP^*1-	Control DNA pUC-BAP^*2100 ng/μL × 50 μL	Dialysis Unit (MWCO: 12～14 kDa)-	Dialysis membrane cup (MWCO: 15 kDa)-	shaker ball-
Product No.A239-0304	Product NameMusaibo Kun N 100 SS	Target ProteinsSecretory Proteins	Additive of membrane proteins◎	Premix reaction solution100 μL reaction × 8	Premix dialysis solution1 mL dialysis × 8	Control DNA pUC-frGFP^*1-	Control DNA pUC-BAP^*2100 ng/μL × 50 μL	Dialysis Unit (MWCO: 12～14 kDa)8 sets	Dialysis membrane cup (MWCO: 15 kDa)-	shaker ball-
Product No.A240-0305	Product NameMusaibo Kun N 1000 SS	Target ProteinsSecretory Proteins	Additive of membrane proteins◎	Premix reaction solution1 mL reaction × 1	Premix dialysis solution10 mL dialysis × 1	Control DNA pUC-frGFP^*1-	Control DNA pUC-BAP^*2100 ng/μL × 50 μL	Dialysis Unit (MWCO: 12～14 kDa)-	Dialysis membrane cup (MWCO: 15 kDa)1 set	shaker ball1 set

^*1 frGFP is synthesized with NHis tag and TEV protease recognition sequence attached to the N-terminus.　

^*2 Escherichia coli-derived alkaline phosphatase (BAP) is synthesized.

^*3 The Musaibo Kun N series cannot be used for the synthesis of stable isotope-labeled proteins.

^*4 The storage temperature of the the Musaibo Kun N series is -80 °C.

You can easily try cell-free protein synthesis. Six reactions of small-scale synthesis (0.1 mL) can be carried out with the included microdialysis cup. The kit is suitable for expression yield/solubility assay and reaction condition optimization.

Product information

Product No: A183-0242

Product Name: Musaibo Kun Start

Pack size: 1 kit

storage temperature: -80 °C

Kit Contents

Musaibo Kun internal solution 180 µL × 3

Musaibo Kun external solution 1.8 mL × 3

Control DNA^※1 (pUC-CAT) 50 µg /mL 50 µL x 1

Unlabeled amino acids mixture^※2 0.6 mL x 1

Dialysis Unit (MWCO: 12~14 kDa) 6 sets

^※1Express chloramphenicol acetyltransferase (CAT) derived from Escherichia coli.
^※2This kit does not include stable isotope-labeled amino acids. Please purchase "Stable Isotope labeled Amino Acid mixture for Musaibo Kun" if you want stable Isotope labeled protein.

This kit can synthesize stable isotope-labeled proteins by combination with various stable isotope-labeled amino acids (sold separately). Up to 5 mg of CAT protein can be synthesized per kit by 16-hour incubation.

Product information

Product No: A29-0059

Product Name: Musaibo Kun SI

Pack size: 1 kit

Storage temperature: -80 °C

^※We can supply polyethylene glycol (PEG)-free kit.
■ Product No: A173-0230
Product Name: Musaibo-kun SI (PEG free)
Please feel free to contact us.

Kit Contents

Musaibo Kun SI internal solution 0.775 mL x 1

Musaibo Kun SI external solution 8.25 mL x 3

Unlabeled amino acids mixture^※1 1 mL x 1

Control DNA (pUC-CAT) 50 µg /mL 50 µL x 1

Dialysis membrane cup 1cup

^※1This kit does not include stable isotope-labeled amino acids. Please purchase "Stable Isotope labeled Amino Acid mixture for Musaibo Kun" if you want stable Isotope labeled protein.

This kit can synthesize stable isotope-labeled secretory proteins such as antibodies with disuffide bonds and cytokines. The oxidation condition can be changed and the kit contains disulfide isomerase.

Product information

Product No: A89-0126

Product Name: Musaibo Kun SI SS

Pack size: 1 kit

storage temperature: -80°C

^※Optimized oxidation conditions differ depending on the type of protein, so please examine the conditions by “microdialysis method” in advance of using. Please contact us for microdialysis cups for examining conditions.
^※We can supply polyethylene glycol (PEG)-free kit.
■ Product No: A241-0307
Product Name: Musaibo-kun SI SS (PEG free)
Please feel free to contact us.

Kit Contents

Musaibo Kun SI SS internal solution 685 μL x 1

Musaibo Kun SI SS external solution 7.25 mLx 1

50 mM Glutathione oxidized form: (GSSG) 1.2 mL x 1

50 mM Glutathione reduced form: (GSH) 1.2 mL x 1

Control DNA^※1 (pUC-BAP) 100 µg /mL 50 µL x 1

Unlabeled amino acids mixture^※2 0.6 mL x 1

Dialysis membrane cup 1cup

^※1Express alkaline phosphatase (BAP).
^※2This kit does not include stable isotope-labeled amino acids. Please purchase "Stable Isotope labeled Amino Acid mixture for Musaibo Kun" if you want stable Isotope labeled protein.

This kit is intended for the advanced confirmation of target protein expression. Proteins can be easily synthesized by adding template DNA and incubating for 1 hour because the reaction solution contains required components for protein synthesis including amino acids.

Product information

Product No: A29-0058

Product Name: Musaibo Kun Quick

Pack size: 1 kit

storage temperature: -80°C

Kit Contents

Quick solution A 0.54 mL x 1

Quick solution B 0.32 mL x 1

Control DNA^※2 (pUC-CAT) 50 µg /mL 50 µL x 1

^※1This kit does not include stable isotope-labeled amino acids.
^※2Express chloramphenicol acetyltransferase (CAT) derived from Escherichia coli.

Additive of membrane proteins

Product No.	Product Name	Pack size
Product No.A226-0290	Product NameMembrane protein expression additive Set A	Pack size1 kit

Stable Isotope labeled Amino Acid mixture for Musaibo Kun

Product No.	Product Name	Pack size
Product No.A107-0144	Product NameStable Isotope labeled Amino Acid mixture for Musaibo Kun-UL-d	Pack size1 mL
Product No.A39-0072	Product NameStable Isotope labeled Amino Acid mixture for Musaibo Kun-UL-¹⁵N	Pack size1 mL
Product No.A41-0074	Product NameStable Isotope labeled Amino Acid mixture for Musaibo Kun-UL-¹⁵N,d	Pack size1 mL
Product No.A40-0073	Product NameStable Isotope labeled Amino Acid mixture for Musaibo Kun-UL-¹³C,¹⁵N	Pack size1 mL
Product No.A42-0075	Product NameStable Isotope labeled Amino Acid mixture for Musaibo Kun-UL-¹³C,¹⁵N,d	Pack size1 mL
Product No.A91-0128	Product NameStable Isotope labeled Amino Acid mixture for Musaibo Kun-Lys,Arg-UL-¹³C,¹⁵N	Pack size1 mL
Product No.A92-0129	Product NameStable Isotope labeled Amino Acid mixture for Musaibo Kun-Lys,Leu-UL-¹³C,¹⁵N	Pack size1 mL
Product No.A108-0145	Product NameStable Isotope labeled Amino Acid mixture for Musaibo Kun-SeMet	Pack size1 mL