quick-lang quick-lang
quick-google quick-google
quick-lang quick-lang
quick-google quick-google

Cell Free Protein Expression kit
"Musaibo Kun"


Cell free protein synthesis is a technology to synthesize proteins in vitro by cell extract supplemented with amino acids, target protein gene, and energy regeneration system. RIKEN's advanced E. coli based cell free protein synthesis technology is now available as Musaibo Kun kits. The kit enables rapid and easy protein synthesis with high isotope labeling efficiency.

* This product was developed with the support of the Japan Science and Technology Agency's "Industry-Academia Joint Seeds Innovation Project."

quick-contact


N-Series

N-Series-logo

We highly recommend these kits for first trial. The kit can express intracellular proteins, secretory proteins, and membrane proteins. Proteins can be easily synthesized by adding template DNA and incubating for 1-16 hours because the reaction solution contains required components for protein synthesis including amino acids.

Product No. Product Name Target Proteins Additive of membrane proteins Premix reaction solution Premix dialysis solution Control DNA
pUC-frGFP*1
Control DNA
pUC-BAP*2
Dialysis Unit
(MWCO: 12~14 kDa)
Dialysis membrane cup
(MWCO: 15 kDa)
shaker ball
A235-0300 Musaibo Kun N Mini Intracellular proteins 50 μL reaction × 18 - 50 ng/μL × 50 μL - - - -
A236-0301 Musaibo Kun N 100 Intracellular proteins 100 μL reaction × 8 1 mL dialysis × 8 50 ng/μL × 50 μL - 8 sets - -
A237-0302 Musaibo Kun N 1000 Intracellular proteins 1 mL reaction × 1 10 mL dialysis × 1 50 ng/μL × 50 μL - - 1 set 1 set
A238-0303 Musaibo Kun N Mini SS Secretory Proteins 50 μL reaction × 18 - - 100 ng/μL × 50 μL - - -
A239-0304 Musaibo Kun N 100 SS Secretory Proteins 100 μL reaction × 8 1 mL dialysis × 8 - 100 ng/μL × 50 μL 8 sets - -
A240-0305 Musaibo Kun N 1000 SS Secretory Proteins 1 mL reaction × 1 10 mL dialysis × 1 - 100 ng/μL × 50 μL - 1 set 1 set

*1 frGFP is synthesized with NHis tag and TEV protease recognition sequence attached to the N-terminus. 

*2 Escherichia coli-derived alkaline phosphatase (BAP) is synthesized.

*3 The Musaibo Kun N series cannot be used for the synthesis of stable isotope-labeled proteins.

*4 The storage temperature of the the Musaibo Kun N series is -80 °C.


START

Start-logo

You can easily try cell-free protein synthesis. Six reactions of small-scale synthesis (0.1 mL) can be carried out with the included microdialysis cup. The kit is suitable for expression yield/solubility assay and reaction condition optimization.

※1Express chloramphenicol acetyltransferase (CAT) derived from Escherichia coli.
※2This kit does not include stable isotope-labeled amino acids. Please purchase "Stable Isotope labeled Amino Acid mixture for Musaibo Kun" if you want stable Isotope labeled protein.


SI-logo

This kit can synthesize stable isotope-labeled proteins by combination with various stable isotope-labeled amino acids (sold separately). Up to 5 mg of CAT protein can be synthesized per kit by 16-hour incubation.

We can supply polyethylene glycol (PEG)-free kit.
■ Product No: A173-0230
Product Name: Musaibo-kun SI (PEG free)
Please feel free to contact us.

※1This kit does not include stable isotope-labeled amino acids. Please purchase "Stable Isotope labeled Amino Acid mixture for Musaibo Kun" if you want stable Isotope labeled protein.


SI-SS

SI-SS-logo

This kit can synthesize stable isotope-labeled secretory proteins such as antibodies with disuffide bonds and cytokines. The oxidation condition can be changed and the kit contains disulfide isomerase.

Optimized oxidation conditions differ depending on the type of protein, so please examine the conditions by “microdialysis method” in advance of using. Please contact us for microdialysis cups for examining conditions.
We can supply polyethylene glycol (PEG)-free kit.
■ Product No: A241-0307
Product Name: Musaibo-kun SI SS (PEG free)
Please feel free to contact us.

※1Express alkaline phosphatase (BAP).
※2This kit does not include stable isotope-labeled amino acids. Please purchase "Stable Isotope labeled Amino Acid mixture for Musaibo Kun" if you want stable Isotope labeled protein.


Quick-logo

This kit is intended for the advanced confirmation of target protein expression. Proteins can be easily synthesized by adding template DNA and incubating for 1 hour because the reaction solution contains required components for protein synthesis including amino acids.

※1This kit does not include stable isotope-labeled amino acids.
※2Express chloramphenicol acetyltransferase (CAT) derived from Escherichia coli.


Product No. Product Name Pack size
A226-0290 Membrane protein expression additive Set A 1 kit

Product No. Product Name Pack size
A107-0144 Stable Isotope labeled Amino Acid mixture for Musaibo Kun-UL-d 1 mL
A39-0072 Stable Isotope labeled Amino Acid mixture for Musaibo Kun-UL-15N 1 mL
A41-0074 Stable Isotope labeled Amino Acid mixture for Musaibo Kun-UL-15N,d 1 mL
A40-0073 Stable Isotope labeled Amino Acid mixture for Musaibo Kun-UL-13C,15N 1 mL
A42-0075 Stable Isotope labeled Amino Acid mixture for Musaibo Kun-UL-13C,15N,d 1 mL
A91-0128 Stable Isotope labeled Amino Acid mixture for Musaibo Kun-Lys,Arg-UL-13C,15N 1 mL
A92-0129 Stable Isotope labeled Amino Acid mixture for Musaibo Kun-Lys,Leu-UL-13C,15N 1 mL
A108-0145 Stable Isotope labeled Amino Acid mixture for Musaibo Kun-SeMet 1 mL
quick-contact
pct_012_d

TAIYO NIPPON SANSO
Corporation.


+81(3)5439-5897
※Mon to Fri
9:00~17:40(JST)


+81(3)5439-5883

Isotope.TNS@tn-sanso.co.jp

Fuji Bldg. 5-30-9 Shiba, Minato-ku,
Tokyo 108-0014, Japan

Taiyo Nippon Sanso's Solution as a Leading Oxygen Manufacture

Copyright © 2018.
TAIYO NIPPON SANSO Corporation.
All Rights Reserved.