18O Labeling reagent "Water-18O"
Proteases (e.g. Trypsin) in Water -18O catalyze 16O/18O exchange at the C-terminal carboxyl group of proteolytic peptides, resulting in 4 Da mass shift between labeled and non-labeled peptide ions observed in mass spectrometry (MS).
This technique has been applied for an absolute quantification method of proteins in the fields of drug discovery and life science .
Taiyo Nippon Sanso provides high quality hydrolyzing 18O labeling reagent "Water - 18O".
Superior Properties of our water -18O
Specification
| Test | Unit | Specification | Method |
|---|---|---|---|
| Oxygen-18O | atom% | ≥98(F03-0027) ≥99(F05-0031) |
MS |
| Oxygen-17O | atom% | <2 | MS |
| Oxygen-16O | atom% | <2 | MS |
| Appearance | - | Clear, Colorless No visible object | Visual check |
| Chemical Purity | % | >99.99 | * |
| Na | mg/L | <2 | ICP-MS |
| Mg | mg/L | <1 | ICP-MS |
| K | mg/L | <1 | ICP-MS |
| Ca | mg/L | <1 | ICP-MS |
| Fe | mg/L | <0.1 | ICP-MS |
| Cu | mg/L | <1 | ICP-MS |
| Zn | mg/L | <1 | ICP-MS |
| NH4+ | mg/L | <1 | IC |
| F- | mg/L | <1 | IC |
| Cl- | mg/L | <1 | IC |
| Br- | mg/L | <1 | IC |
| NO3- | mg/L | <1 | IC |
| PO43- | mg/L | <1 | IC |
| SO42- | mg/L | <1 | IC |
| I- | mg/L | <1 | IC |
| Total Oganic Cabon | mg/L | <5 | TOC analyxer |
| Conductivity | mS/m | <0.3 | Comductivity meter |
| pH | 5.5-8.0 | pH meter | |
| Microbial limit | cfu/mL | <1 | Membrane filter |
| Pyrogenicity | EU/mL | <0.25 | LAL |
Oxygen-18O Labeling
Taiyo Nippon Sanso supplies building blocks for Oxygen-18O labeling and offers a custom synthesis service of 18O-compounds that are not commercially available.
18O-assisted mass spectrometry has advantages over conventional D, 13C and 15N labeling .
18O introduction reagent（>95atom% 18O）
By using 18O introduction reagent, any compound can be labeled with 18O.
PRODUCT No. : A102-0139
PRODUCT : Ethoxycarbonylmethyl(triphenyl)phosphonium-18O Bromide 98atom% 18O
PRODUCT No. : A100-0137
PRODUCT : 2-Amino-5-phenyl-1,3.5-Oxadiazole-18O 95atom% 18O
PRODUCT No. : A106-0143
PRODUCT : Dirthyl Carbonate-18O 98atom% 18O
PRODUCT No. : A104-0141
PRODUCT : Uracil-18O2 95atom% 18O
PRODUCT No. : A105-0142
PRODUCT : 5-Fluorouracil-18O2 95atom% 18O
TAIYO NIPPON SANSO
Corporation.
+81-4-5872-1823
※Mon to Fri
9：00～17：40(JST)
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Isotope.TNS@tn-sanso.co.jp
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4-6-2, Minatomirai, Nishi-ku, Yokohama,
Kanagawa 220-8561, Japan
Taiyo Nippon Sanso's Solution as a Leading Oxygen Manufacture
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